The confirmation test is always necessary due to the test on specific substances.Immunoassay test is the technique that identifies and measures the level of a drug or poison from a sample taken from an individual. The technique uses the principle of chemical reaction of antibodies to their specific antigens. The test is common due to the ability to test and determine the level of concentration of the drug or detected poison in the isolated sample (Flanagan, Perrett and Whelpton, 2005). Antibodies that are used in the test are obtained from animals through combination of the drug or poison with a protein which results to the production of complex of drug and protein. The substance is then injected in the animal where it is apparent in the immune system of the animal as an antigen (Bell, 2009). The immune system of the animal injected will produce specific antibodies which will act against the complex. The antibodies are then extracted from the blood of the animal for use in the test. Immunoassay testing is always followed by a confirmation test due to production of a false positive result which is caused by organic compounds with similar chemical structure to drugs and poisons.Gas chromatography is a technique used in identification of drugs and poisons through the separation of an isolated drug or poison to characteristic component chemical portions. The technique is common due to the accuracy and it being fast. In carrying out the test, the sample that is isolated is placed in a chamber with high temperatures and nitrogen gas passed through it. Isolated sample is then made to pass through the tubing with the components completely separating. Toxicology, Drugs and Poisons.
Bell, S. (2009). Drugs, poisons, and chemistry. New York: Facts On File, Inc. http://resource.rockyview.ab.ca/t4t/forensicscience35-5cr/Module06/L3/M6L3P02-Extraction.html
Flanagan, R., Perrett, D., & Whelpton, R. (2005). Electrochemical detection in HPLC. Cambridge, UK: Royal Society of Chemistry. http://all-about-forensic-science.com/forensic-toxicology.html
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