The treated cells were used as donor nuclei for nuclear transfer. Te transferred cell was passed through electric pulses that were an activation factor. Tis gave it a high multiplication rate of 62. 5 twelve hours after activation. Te nuclear transfer and exposure to electric pulses led to condensation of chromosome by 0. 5 due to the exposure of the cell to the colchicine solution, mst of the embryos were diploid. Ater two hours of the fusion, tere was detection of very strong fluorescence in most of the Tese reconstructed embryos later developed to blastocyst phase that most of them expressed Enhanced Green Fluorescent Protein.
Tese embryos were later transferred into surrogate gilts that out of four of them, tree were found to be pregnant. Oe of the pregnant surrogates gave birth into the healthy piglets with characteristics of showing that they contain Enhanced Green Fluorescent Protein. Pytase that is very much effective in digestion of phytate is expressed in the salivary glands parotid secretory organs of transgenic pigs. The salivary glands are found in the mouth mouth.
Te scientist made sure that phytase is produced early enough during the start of digestion so that to give it time for distention as food moves down the intestines. Hw they made it: i was developed by introducing the transgene pigments which were composed of some promoters elements of murine parotid secretory protein gene a bacterial by the name Escherichia coli which is responsible for phytase genes (Golovan et al, 167). Tis changed its genetic characteristics into a fully fertilized porcine embryo by pronuclear. Te embryo together others through surgical method implanted into reproductive track of a uterus synchronized female.
Te pigs have shown some good characteristics of well-growing habit. Tey needed little-supplemented feed as compared to the Yorkshire breed. Cmpared to Yorkshire, tey could eat the same diet but more supplemented with P for Yorkshire. Hwever, tere have been some emerging problems on their growth. Fr example, abreed from transgenic has shown characteristics of weak legs. Tis is the effects of over expression of the genes. Te same fact has caused weaknesses in the system of breeds (RosENGARD 207).
O the other hand, uder expression of the genes has. ..
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