Differential diagnosis could be replaced by reverse transcription polymerase reaction, DA sequencing and DNA microarray. Qantitative real time reverse transcription polymerase chain reaction (RT-PCR) can be used for differential expression of genes in HGDN and WD-HCC. Tis method is fast, hghly sensitive and specific. Te starting material could be liver biopsy or surgical specimen. Ientification of lymphomas by demonstration of presence or absence of clonality is done suitaby using PCR. Te most efficient markers of HCC are used to establish a specific molecular diagnostic index. The selected genes most relevant metabolic pathways involved in liver carcinogenesis and are regulated at the transcriptional level (Paradis, e al.
Qantitative real time PCR measure microMRNA which make useful biomarkers in differential diagnosis of HCC. DA microarrays is important in distinguishing different histological grades of HCC, ad genetic profiles associated with venous invasion and metastasis. Te microarray technology enables identification of specific gene expression patterns that distinguish HCC. Hp 70 and Hsp 90 families are molecular chaperones that regulate many key components of apoptotic pathways and molecular chaperones. T clarify the associated with the multistep process, te gene expression of 7 early components and 7 progressed components of nodule in nodule type HCC are analyzed.
Hp 70 in particular is a sensitive marker for the differential diagnosis of early HCC. Te expression of Hsp 70 increases in early and progressed HCC (Chuma, e al. Gnes that were classified in the immune system detoxification category were down regulated in carcinogenic cells. I the early stage of HCC development, imune surveillance deteriorates and multiple apoptosis and histocompatibility complex related genes were regulated the transition of liver cirrhosis to GI or GII HCC.
Tere are a series of genes classifiers with an accuracy of 95% distinguishing LGDN and HGDN. I addition, tese gene classifiers can effectively differentiate between GI-GII and GII-GIII HCC. I moderately to poorly differentiated HCC, te reduced rate of apoptosis could indicate major tumor progression characteristic (Maass, e al. DA sequencing is a non-invasive, erly detection and differential diagnosis of HCC. Te method has. ..
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