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Lactase Enzyme Activity

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Based on my knowledge of the effects of temperature and pH on the enzymatic activity of lactase, if the lactase used in today’ s lab was extracted from human cells, I hypothesize that lactase enzyme activity will be optimal at temperature and pH of 400C and 8 respectively. Microfuge tubes were selected and labeled according to the selected temperatures 00C, 250C, 400C, 600C, 800C and 1000C and each filled up with 0.5 lines with lactase solution. The solutions were then maintained at water baths with respective temperatures for five minutes after milk was added to lactose solution up to 1.0 line using an alternate plastic pipette.

The solution was left for ten minutes after which a glucose strip was placed into each of the test tubes for one second and allowed to sit on the bench for thirty seconds. The coloration of the glucose strip was then compared with the chart and the amount of glucose recorded in mg/dL. Seven microfuges were selected and labeled; 2, 4, 5, 6, 7, 8, 10, and 12, filled with appropriate pH buffer up to line and 3 drops of milk added into each tube.

The solution solutions in the seven tubes were then mixed by inverting the tubes three times after which 3 drops of lactase solution was added to each tube using a plastic pipette. All tubes were then incubated for 10 minutes in a water bath at 400C. A glucose strip was then placed in each test tube for one second, removed and left on the bench for thirty seconds and finally coloration compared to the chart provided. Two microfuge tubes were and labeled “ L” and the other labeled “ M” .

Lactose and Maltose solutions were then added into the tubes labeled L and M respectively up to the 0. Lactase solution was then added into each tube up to 1.0 line and the mixture placed in 400C water bath for 10 minutes. After ten minutes, a glucose strip was then placed into each tube for one second, removed and placed on the bench for 30 seconds and coloration finally compared to the chart provided and amount of glucose determined recorded in

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