The diagnostic methods can be divided into several subsections that include laboratory methods, atigen detection methods, ad nucleic acid amplification methods (McFarland, 2011, p The laboratory methods include culture Agar media, eriching pores, CN test, ad selective media. Te antigen detection methods, o the other hand, iclude GDH Assays while the nucleic acid amplification methods include PCR Assays and LAMP Assays. Athough there may be many diagnostic methods for detecting C. dfficle, te most effective and the ones that are used in the contemporary world include culture, ctotoxic immunoassay, ltex agglutination, plymerase chain reaction (PCR), ad loop mediated isothermal amplification or LAMP.
Ech of these diagnostic methods detects varying signs or symptoms for determining the existence of C. I addition, tey have advantages and disadvantages, wich may make some desirable as compared to others. Aong the diagnostic methods, clture has been the most common for a long time. Clture is mostly used to detect toxigenic and nontoxigenic C. Tis implies that culture can detect two forms of the infection with accuracy. Te advantages associated with culture method it is sensitive and it allows strain typing in the epidemics.
Hwever, i requires aerobic culture. I is not precise for toxin producing bacteria. I addition, i takes some time to get the result. Secifically, i takes between 2 and 5 days to receive the results. Clture has been seen to detect additional 15% of Clostridium difficle cases mostly those that other methods such as direct toxicity neutralization assay or DCNA have missed. Athough culture has been able to detect additional changes of infection and been utilized extensively the and 80s, fw laboratories continue to utilize culture based methods of diagnosis.
Acording to recent study and research indications, mst of the diagnostic methods in use today are non-culture based. Tis method was being utilized extensively in the past due to the advantage of being sensitive (Kuijper & Wilcox, 2008, p However, cntemporary research has shown that in spite of this method being sensitive, i is not specific for diagnosis. Tis is because the nontoxigenic strains linked to C. dfficle can be retrieved from samples of stool. strains are not to be pathogenic.
Nvertheless, clture, de to its. ..
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