Such a trait or genetic characteristic for example may be the trait of drought resistance or resistance to attack by insects. Tis step is followed by insertion of the isolated gene of interest into a selected vector and in most cases the vector used is the bacteria Agrobacterium tumefaciens. Te gene insert that contains the gene of interest is inserted into the plasmid of the vector by use of an alternative in the various methods of recombinant DNA technology (Grundey & Rimkiene, 2012). Te already genetically modified Agrobacterium properly mixed with plant cells or parts of the plant such as the leaves or stems for purposes of plant transformation.
Nt all plants cells that take up the transferred DNA in this case referred to as the T-DNA but at least most of the plant cells manage to incorporate the gene of interest into their own genome (MacDonald, &Whellams, 2007). Tmifaciens then manages to inoculate the genes of interest into the transgenic plant cells which now become genetically engineered. Agood example of a technique that can be used the gene of interest into the transgenic cells is by use of a gene gun that bombards small particles coated with the gene of interest into the transgenic cells (De mirci, 2008).
Te step that follows is whereby the modified plant cells or the cells that took up the gene of interest are identified. Tere are lots of cells that indeed fail to take up the gene of interest and they must be differentiated from those cells that took up the gene of interest which are necessary for next Tis is achieved through the use of selectable markers in which the cells are marked with genes (alongside genes possessing desired characteristics) expressing the trait or growing in the presence of herbicides or antibiotics.
Te cells that did not take up the gene of interest fail to grow under such conditions. Tose cells that grow in media containing certain antibiotics or herbicides whose resistance genes are included into the gene insert are correctly assumed to have taken the gene of interest (Daboub et al. Wat then follows that the modified plant cells are cultured into whole organisms, i this case whole plants, trough various tissue culture techniques.
Tis requires placing the plant cells (that managed to form colonies in media. ..
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