The washing detergents were Suma Nova L6 t and Suma Select A7 maintaining a 10 - 11. Te eggs were later on transported for cuticle staining for the assessment of their ultra structure (Leleu et al 1650). Te cuticle staining took place for 100 eggs from each type of egg both washed an unwashed. T stain an egg, i was immersed in an aqueous solution for 1 min. te solution contained 2. 8g of Green S and 7. 2g of Tartrazine. Bfore drying, te eggs were rinsed to dye.
Acalorimeter was responsible for quantification of the eggs by measuring the differences in the colors of the eggshells at four different points around the egg’s equator before and after the staining process. For the ultra structural assessment, 30 eggs were selected from each group. Acircular saw that had a diamond tip aided in removing a 1-cm section of the eggshells around the equator. Te sections were viewed using Scanning Electronic Microscopes (SME). T assess the cuticle coverage, te researchers performed a two-sample t test that was one-sided (Leleu 1651).
Ater the cuticle staining, al the calculations for both the white and brown eggs showed no statistical difference between washed and unwashed eggs. Fr the ultra structural assessment, te calculations also showed no significant difference between washed and unwashed eggs of either the brown or the white eggs. Tere was even cuticle coverage in 13 of the eggs tested, i which most were brown. I around 56. 6 to 83. 3 of the eggs, tere was occasional or very patchy cuticle coverage. Te pores were visible in 3 76.
7 of all the eggs assessed. Amost one of the eggs tested had been contaminated heavily with debris. SE allows the shell damage from washing to be critical appraised. I most of the eggs assessed, 63. 3 to 86. 7), tere was no cuticle damage. Te white and brown eggs were distributed proportionally in all categories and there was no statistical difference between the washed and the unwashed eggs ((Leleu et al 1651). Te eggs collection was purposely from old laying hens because cuticle thickness decreases the hen to age and the study needed eggs with the worst-case scenario possible.
Te researchers considered the cuticle coverage poor regardless of origin or treatment. Te exposed pores in the study were because there were no cuticles in the beginning. Een in cases where the cuticle was present, tere were no. ..
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