On the other hand, proteins which facilitate the binding of RNA polymerase are activators. T5hese activator proteins bind to -35 region that is present in the flanking region or even further ahead enhancing the process of transcription. Prokaryotic transcription begins with the unwinding of the double-stranded SNA. This unwinding is induced by the binding of the RNA polymerase to the promoter area. The sigma factor comes into play when the RNA transcript has ten nucleotide bases. The transcription only stops after a termination signal is encountered by the RNA polymerase. The prokaryotic transcript is a polycistronic transcript. A polycistronic transcript is one which on being translated gives a great number of different proteins. The process of transcription is a complex process and the regulation of it has been presented. In the prokaryotes, there are other two regulatory mechanisms which can further explain the control of transcription in these prokaryotic organisms. These two mechanisms are termed as positive control and negative control. The functioning of these control mechanisms will be considered with examples. An operon which is a genetic unit that consists of genes that produce a number of genes works in the control of prokaryotic transcription. The operon codes for a polycistronic transcript which codes for multiple proteins. These proteins may be enzymes which function in collaboration. The Process of Transcription and the Generation of Proteins.
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