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Cell signalling coursework Essay Example

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Cell signalling coursework

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When they are activated, the G protein exchanges GDP for GTP. As a result of this dissociation of the GTP-bound α and β/γ subunits, a number of diverse signalling cascades are triggered. Receptors attached to diverse heterotrimeric G protein subtypes can use different scaffolds to activate the small G protein/ MAPK cascade such as Tyr kinases. GPCRs may also utolize PLCβ as a mediator in the activation of PKC and CaMKII, which can either stimulate or inhibit the MAPK pathway downstream.In the experiment above EPGF was used as a positive control since the HTl cells normally over express HER2 which is an EGF receptor. The negative control consisted of untreated cells which lacked the external stimuli to induce signal transduction thus there was limited proliferation. On the basis of the evidence in fig 1 and using a diagram, identify the pathway(s) that is (are) most likely to lead to cell proliferation following treatment with the MF8 ligand. Give clear reasons for your answer.Additionally, proliferation in the presence of MF8 was observed in H89 which is a PKA inhibitor. However, signal transduction will not stop since PKA and EPAC are responsible for activation of RAP-1 thus it continues to proliferation stage. Proliferation was also observed in PMA due to its ability to induce PKC activation thus continuation of the signal transduction.On the other hand minimal proliferation was observed in the PD98059 which is an ERK inhibitor. This is due to the fact that when ERK is inhibited it will not enter the nucleus where transcription occurs. It was also observed that little proliferation was recorded in the PKC inhibitor. This is because it inhibited RAS GAP which in turn inhibits Rasthus stopping the signalling process.In addition to the PCR/Western blot techniques flow cytometry can also be used to analyse signal transduction pathways in culture cells by detecting phosphor-epitopes. The Stat5 phoshorylated on tyrosine can be used as a cell based measure of Bcr/Abl activity in chronic myeloid leukemia cell line signalling. This experiment can be repeated with kinase inhibitor such as STI571. This kinase inhibitor blocks transcription thus preventing proliferation of

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preview essay on Cell signalling coursework
  • Pages: 8 (2000 words)
  • Document Type: Essay
  • Subject: Biology
  • Level: Undergraduate
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