A condenser lens is used to direct light rays from an illumination source to the specimen that is bound to get examined.Condenser lenses are most useful at the highest powers (400X and above). Microscopes with in stage condenser lenses render a sharper image than those with no lens (at 400X). If your microscope has a maximum power of 400X, you will get the maximum benefit by using a condenser lenses rated at 0.65 NA or greater.65 NA condenser lenses may be mounted in the stage and work quite well. A big advantage to a stage mounted lens is that there is one less focusing item to deal with. If you go to 1000X then you should have a focusable condenser lens with an N.25 or greater. Most 1000X microscopes use 1.25 Abbe condenser lens systems2. The Abbe condenser lens can be moved up and down. It is set very close to the slide at 1000X and moved further away at the lower powers.The appropriate method to focal point a microscope is to begin with the lowly power objective lens and as one is observing from a specific region, eccentric the lens downward as near to the sample as possible devoid of moving it. After that, glance using the eyepiece lens and spot increasingly until the specimen is sharp. In case there happens to appear some problems during the focus, a repeat of the whole process is supposed to be done. As soon as the image is sharp with the low power lens, an individual should be able to adjust in the next power lens and do slight changes with the focus handle. If the microscope is fitted with a fine focus adjustment, rotating it would be enough to achieve great images.After the experiment, switch off the microscope so as to return it to its storeroom. Position the low power objective in place and lower the body tube. Envelop the scope with the dust cover and place it in its usual place3.Mitosis is best observed in cells that always have rapid growth as in the onion root cell tips. This is because the root contains a special growth region commonly referred to as the apical meristem whereby high percentage of cells are believed to undergo mitosis4. Onion cells are in general rectangular in shape where they mostly range from a quarter to a half millimeter. To be able to observe chromosomes properly, it is essential to ensure that the microscope used is of high quality and has high magnification power. This is because the observation of the chromosomes in the nucleus requires that one has their
Borowick, Jerome N. lab report. Chicago: Prentice Hall, 2000.
D., Andrew. Mitosis: Methods and Protocols (Methods in Molecular Biology). Chicago: Humana
Dawes, Clinton J. Introduction to biological electron microscopy: Theory and Techniques.
Washington: Ladd Research Industries, 1988.
Egerton, R.F. Physical Principles of Electron Microscopy. New York: Springer publishers, 2010.
French, Marvin L. The Human Cell. New York: Marvin L French publishers, 2011.
Gilbert, Scott F. Developmental Biology. Columbia: Sinauer Associates, 2010.
Hayat, M. A. Introduction to Biological Scanning Electron Microscopy. Chicago: University
Park Press, 1992.
Matsudaira, Paul. Molecular Cell Biology. New York: W. H. Freeman, 1999.
Najjar, V.A. Enzyme Induction and Modulation. New York: Springer, 2000.
R.C, Elizabeth. All About Mitosis and Meiosis: Life Science. Washington: Teacher Created,
Schrader, Franz. Mitosis: The movements of chromosomes in cell division. Columbia: Columbia
University Press, 1944.
Sullivan, Mary. All About Mitosis and Meiosis (Mission: Science) . New York: Compass Point
Please type your essay title, choose your document type, enter your email and we send you essay samples