Light microscopy on the specimen - The light microscope was set and used it to examine the slide. Te nucleic was observed to be blue and cytoplasm red. The images observed under the microscope were drawn, ad the following components were labeled: Endometrial glands, Stroma and Myometrium. Oe advantage of preparing frozen section is that, i can be prepared first as compared to other staining methods. Te frozen sections are largely used to examine tissues in during surgery. For instance, during the diagnosis of aoasophagus tumors, fozen section help to define resection margins for pastroesophageal resection.
Moreover the method helps to evaluate the extent of disease spread. I such a case, fozen section may aid in complete resection of the tumors cell (Kim, &Weickert, 2007). Ech step introduces artifacts by distorting the cell natural appearance. Some artifacts are avoidable while others are unavoidable. Aong the most common artifacts are wrinkles and scratches. Sratched are caused by dirt on the cutting edge. Te procedure should be carried out with care to avoid unintended artifact since they can result to wrong diagnostic.
Unintended artifact can be minimized by optimal procedure. Fom the examination of the uterus tissue sections, sme endometrial gland contained narrow lumen. Tis was an indication of folical phase. The luteal phase was also observed (Weickert, 2009). A the beginning of the phase, pogesterone stimulates secretion osecretef mucus and glycogen by the endometrial glands. Te large lumens are, terefore, a a result of increased secretary activity. H& E is the most often used staining method in the histology lab for tissue diagnostic. H&E is mostly used as it provides pathologist a very detailed view of the tissue (Kiernan, 2009).
The method achieves this by clearly straining cell structure. Sme of the cell components strained is nucleus, ctoplasm, oganelles, ad extra cellular components. The information from HE& E is sufficient to allow diagnosis of disease and to show abnormalities. I can also show particular indicators of abnormalities such as nuclear changes common in cancerous cells. Erors are occurring during the staining lead to false diagnostic. Msmatching is one of the common errors that occur during staining. I may result from, mxing of specimens.
T avoid mismatching, i is important to clearly label all specimens from the
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