Bacteria isolated from vaginal swab 2 (V2) exhibited a positive gram reaction and was found to be cocci dividing into multiple planes. This is indicative of a Staphylococci (Kloos & Schleifer, 1975). Further, the irregular, curled, raised, medium and yellow colonies are suggestive of Staphylococcus aureus. The yellow colour of the colony rules out Staphylococcus epidermidis which has white colonies (Sandwik & Brown, 1965). Next, V2 shows alpha haemolytic activity, which again suggests it to be Staphylococcus. aureus, since none of the other staphylococci, shows haemolytic activity (Marcus et al. , 1997).
It shows no change in colour on MacConkey agar, which further substantiates the conclusion that it is a gram-positive bacteria (Fluornoy, 1900). Growth on mannitol agar is indicative of V2 being either a Staphylococci or a Micrococci and the colour change of the media is indicative of coagulase-positive staphylococci, S. aureus (Koch, 1942). Next, a positive catalase test rules out the possibility of V1 being streptococci or enterococci, which are catalase-negative (Taylor & Achanzar, 1972). Thus on the basis of V2 having the capacity to ferment mannitol it can safely be concluded that it is S.
aureus. S. aureus is a normal flora found on the skin of humans and infection occurs only in cases of invasion through a lesion present on the skin due to previous trauma. However, S. aureus has been isolated from Urinary tract of humans (Muder et al. , 2006), and has been found to be a cause of symptomatic UTI in some cases. S. aureus is resistant to most antimicrobial agents: ampicillin, chloramphenicol, tetracycline, cephalothin, clindamycin and can only be treated with gentamycin. FAECAL SAMPLE (F): Bacteria isolated from the faecal sample (F), showed a gram-negative reaction and is also a bacillus.
This result excludes the gram-positive and the cocci. The colonies are round, flat, entire, small and cream, which is suggestive of bacteria being either coliform or Salmonella.
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